JEREZ Susana Josefina
congresos y reuniones científicas
The excess of fat in the diet would inhibit oral administration-effects of zuccagnia punctacta extract on the alterations induced by hypercholesterolemia
AGOSTINA VALOY; JULIETA ROCO; CATIANA ZAMPINI; MARIA INES ISLA; MIRTA MEDINA; SUSANA JEREZ
Jornada; Jornadas de la Asociación de Biología de Tucumán; 2021
Oral administration of 2.5 mg EAG / day of a standardized extract of Zuccagnia punctacta (E-Zp)has lipid-lowering effects and improves vascular function in an experimental model ofhypercholesterolemia. Our objective was to evaluate the effects of the oral administration of E-Zpin an animal model of high fat and high cholesterol diet -induced insulin resistance associated withhypercholesterolemia. Methods: Male rabbits were separated into 4 groups: 1) fed on regular diet(CD); 2) fed on diet rich in 18% fat and 0.3% cholesterol (M); 3) fed on M orally administered 2.5mg EAG / day of E-Zp; 4) fed on M orally administered 2.5 mg/day of ezetimibe (NPC1L1 inhibitor).After 6 weeks of treatments, biochemical, clinical and vascular parameters were analyzed.Results: The M group, compared with the CD animals, showed abnormal glucose and insulintolerance curves, increased total cholesterol (TC) and visceral abdominal fat (VAF) values,decreased HDL-C, and acetylcholine, angiotensin II and norepinephrine responses. Theadministration of E-Zp did not modify any of these alterations. Ezetimibe treatment significantlydecreased TC and normalized relaxation to acetylcholine and response to norepinephrine.However, it did not modify VAF or glucose intolerance. In conclusion, these preliminary resultssuggest that the lipid-lowering mechanism of E-Zp action would not involve the cholesteroltransporter NPC1L1. We hypothesized that E-Zp would act as a competitive inhibitor of pancreaticlipase, which is why dietary fats interfere with its effect.