IFEC   20925
INSTITUTO DE FARMACOLOGIA EXPERIMENTAL DE CORDOBA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Comorbidity Between Chronic Restraint Stress and Cocaine Self- Administration: Role of Glial Proteins in Nucleus Accumbens Plasticity
Autor/es:
GUZMAN ANDREA SUSANA; BOLLATI FLAVIA; SANCHEZ MARIANELA ADELA; EULIARTE PIA; CANCELA LILIANA M; AVALOS MARIA PAULA; RIGONI DAIANA
Lugar:
Cordoba
Reunión:
Congreso; 2018 Meeting of Argentine Society for Research in Neurosciences; 2018
Institución organizadora:
Sociedad Argentina de Neurociencias
Resumen:
Comorbidity Between Chronic Restraint Stress and Cocaine Self-Administration: Role of Glial Proteins in Nucleus Accumbens Plasticityglutamatergic innervations to Nucleus Accumbens (NA) in animal models. Ourpreviews studies revealed chronic restraint stress-induced increase in basalglutamate (GLU) levels- and decrease in GLU transporter, GLT-1, levels in NA core.These results have been suggested as a hallmark of the homeostatic disruption ofGLU transmission and have been associated with the facilitation of cocaine selfadministration (SA) induced by stress. In parallel, our data showed chronic stressinduced enhancement of TNF-α mRNA levels in NA core after a cocaine challenge. Moreover, all these alterations were prevented by minocycline, a potent inhibitor of microglia activation, suggesting the participation of these cells in the studied phenomenon. In the present project, we propose the increase of TNFα and activation of NF-kB as key contributors of the stress-induced dysregulation of GLU homeostasis, post-synaptic changes and cocaine SA. Thus, rats will be administrated with lentiviral vectors targeted TNFα or NF-kB in NA core and a cocaine SA model will be used to evaluate: 1)Glial modulation of TNFα/NF-kB on GLU homeostasis, 2)GLT-1 levels, 3)currents mediated by AMPA and NMDA receptors and morphology of dendritic spines. In this way, we put forward the use of gene manipulation techniques in order to deepen the study of the underlying neurobiological basis of the comorbidity between stress exposure and cocaine abuse.