IFEC   20925
INSTITUTO DE FARMACOLOGIA EXPERIMENTAL DE CORDOBA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Cocaine decreases ABPs phosphorylation and increases AMPA surface expression in rat nucleus accumbens following chronic stress
Autor/es:
M. ALEJANDRA ESPARZA ; GARCIA KELLER, C; VIRGOLINI, M; CALFA, G; KALIVAS, P.W; CANCELA L.M
Lugar:
Valparaiso
Reunión:
Workshop; II International Workshop. Motivated behavior, stress and addiction. From Molecules to Behavior; 2012
Institución organizadora:
LARNEDA
Resumen:
Behavioral sensitization is an example of experience-dependent plasticity, induced by drug or stress, which has been suggested to involve plasticity at glutamatergic synapses and there is evidence for a common mechanism triggered by stress and drugs at excitatory synapses on midbrain dopamine neurons. These experiments evaluated how the expression of stress-induced sensitization to cocaine (15 mg/kg i.p.) is associated to alterations in actin binding proteins (ABPs) and the surface expression of GluR1 in nucleus accumbens (NAc). Male wistar rats were restrained daily (2 hours) for 7 days, and three weeks later, the NAc was dissected 45 min following acute saline or cocaine (30 mg/kg i.p. or 15 mg/Kg). F-actin, ABPs and AMPAR were determined by western blotting. Locomotor activity was monitored in a photocell apparatus and quantified as total photocell counts. For these sessions, animals were allowed to habituate to the activity chambers before latrunculin A or DMSO (1%) microinjections, which were followed by cocaine (15 mg/kg, i.p.) or saline. Our experiments revealed a decrease in p-cofilin and p-cortactin, and an increase in GluR1, in the stress plus cocaine group as was previously shown after cocaine (30 mg/kg). The stress-induced sensitization to cocaine was prevented by either latrunculin A or CNQX. Interestingly, latrunculin A also reversed the stress/cocaine-induced increase in GluR1. This study shows that a history of repeated stress alters the ability of a subsequent cocaine injection to modulate the reorganization of synaptic connections, actin cytoskeleton and AMPAR expression in the NAc.
Grants: FONCyT, SECyT, CONICET