Red wine polyphenols synergistically inhibit angiotensin II-induced oxidative stress in mesenteric muscle cells

REDONDO,ANALIA; CASTRO CLAUDIA; ESTRELLA, NATALIA; GARCIA, MIRIAM; CRUZADO MONTSERRAT

Mendoza

Jornada; XXII Jornadas de Investigación y IV Jornadas de Posgrado de la Universidad Nacional de Cuyo y 28° Reunión Científica anual de la Sociedad de Biología de Cuyo; 2010

UNCuyo y Sociedad de Biología de Cuyo

Dietary polyphenols are associated with the prevention of hypertension. Angiotensin II (AII) is an important humoral factor associated with this disease. Reactive oxygen species (ROS) are involved in AII-induced vascular action. NADPH oxidase is the major source of vascular ROS. We studied the effect of antioxidants present in red wine, quercetin (Q) and catechin (C), on AII-induced ROS generation in vascular smooth muscle cells obtained from mesenteric arteries of spontaneously hypertensive rats (SHR). ROS production was evaluated by incubation with a fluorescent probe (H2DCF-DA) and NADPH oxidase activity by chemiluminescence with lucigenin. Data (mean±SEM) were analyzed by ANOVA and Bonferroni’s post test. AII (10-7M) increased NADPH oxidase activity (174%±20 vs.100%±10). Q (5µg/mL) inhibited AII effect, instead C (6µg/mL) had no effect. Similarly AII-induced ROS production was inhibited by Q, and C had no effect. Coincubation Q plus C significantly inhibited AII-induced ROS generation (2,43 URF±0,33 vs. 0,60 URF±0,30; **P