Expression and modulation of prolactin receptor in immune cells: Induction of mRNA and proteins by dexamethasone

NEIRA, FLAVIA JUDITH; VALDEZ SUSANA RUTH; MACKERN-OBERTI, JUAN PABLO; SÁNCHEZ, MARÍA BELÉN; PIETROBON, ELISA OLIVIA; JAHN, GRACIELA ALMA; MORENO-SOSA MARÍA TAMARA; SOAJE, MARTA; RIVERO VIRGINIA E.

Buenos Aites

Congreso; Reunión de las Sociedades de Biociencias 2020; 2020

SAIC-SAI-SAFIS

It is known that there is a relationship between hyperprolactinemiaand autoimmunity. However, the role of prolactin receptor (PRL-R) in autoimmunedevelopment is still controversial. The aim of this work is to analyze theexpression of PRL-R in murine immune cells, and determine whether PRL-R aremodulated during stimulation with Concanavalin A (conA) and suppression withDexamethasone (dexa) in healthy mice and three mice models for Lupus. For thisend, splenocytes from C57BL/6 (C57), and lupic FcgRIIb KO (RIIb), NZM, andMRL/MpJ-Faslpr (FAS) mice were stimulated with 1 µg/ml of conA or 1 µg/ml dexa andkept in culture for 24 hours. RNA from splenocytes was isolated using Trizoland retrotranscribed to cDNA to detect PRL-RL by qPCR. Protein determinationwas carried out by flow cytometry using Anti-CD4, Anti-CD19, and Anti-PRL-R. Wefound that splenocytes from RIIb mice have higher mRNA of PRL-RL expressionthan other strains. In CD4+ T cells from C57 mice PRL-R increased by suppressionwith dexa and did no changes when stimulated with conA. In contrast, in CD4+ Tcells from FAS mice, PRL-R increased after conA and decreased with dexa, while theCD4+ T cells from RIIb and NZM mice displayed no changes. In CD19+ B cells fromC57 and FAS mice, we observed an increase of total PRL-R when we stimulatedwith dexa and no changes with conA, while in CD19+ from RIIb and NZM mice, didnot display changes.  These results showa differential behavior of PRL-R expression in C57 mice compared with the lupicmice after stimulation with conA and dexa. The factthat C57 displayed low levels of PRL-R during activation but high insuppression gives us the notion that PRL-R may contribute to maintaining acellular immune balance by limiting PRL trophic effects. By contrast, the highlevels of PRL-R during activation in FAS mice suggest an exacerbated trophic effectof PRL. Our data show that differential PRL-R expression in immune cells may contributeto regulate the immune response.@font-face{font-family:Arial;panose-1:2 11 6 4 2 2 2 2 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536859905 -1073711037 9 0 511 0;}@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1073786111 1 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin-top:0cm;margin-right:0cm;margin-bottom:10.0pt;margin-left:0cm;line-height:115%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:Calibri;mso-fareast-font-family:Calibri;mso-bidi-font-family:"Times New Roman";mso-ansi-language:ES-AR;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:10.0pt;mso-ansi-font-size:10.0pt;mso-bidi-font-size:10.0pt;font-family:Calibri;mso-ascii-font-family:Calibri;mso-fareast-font-family:Calibri;mso-hansi-font-family:Calibri;}div.WordSection1{page:WordSection1;}