IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Hyperthyroidism increases milk immune cells and impairs offspring development in early lactation
Autor/es:
MORENO SOSA TAMARA; SOAJE M; NEIRA F; VALDEZ S; SANCHEZ B; PIETROBON E; MACKERN-OBERTI, JUAN PABLO
Lugar:
mar del plata
Reunión:
Congreso; SAIC-SAFE-SAB-SAP; 2020
Institución organizadora:
SAIC
Resumen:
Hyperthyroidism (H) reduced milk ejection and quality, impairing maternal behavior and mammary gland development. However, it remains unclear if H impacts in milk immune cells numbers. Our aim is to assess the influence of H on i) pup maturation and development ii) prolactin secretion and iii) milk immune cells. For this purpose, 10-12 weeks old Wistar rats were injected daily with T4 (0.25 mg/kg until day 18 of gestation, then 0.1 mg/kg until day 2 of lactation L2) to induce H or with vehicle in control group. Rats were mated 8 days after starting T4 treatment and euthanized L2 (after ketamine/xylazine sedation and oxytocin stimulation for milking). Afterwards, milk and mammary gland samples, minced to reach single cell suspension, were dyed with fluorophore labeled mAbs (CD45+, CD3+, CD11b/c+) and analyzed by flow cytometry. Offspring weights on L1 and 2, head circumference and body length (L2) were measured. Serum of dams and offspring was obtained to determine total T4 and prolactin levels by RIA. Our results show that H mothers had more implantation sites and pup number (p