Comparative response of breast cancer cell lines to treament with Tessaria absinthioides aqueous extract and commercial chemotherapics.

SOSA LOCHEDINO A; HAPON MB; RINALDINI E; GAMARRA LUQUES C; TRONCOSO ME

San Luis

Congreso; XXXVII Reunión Anual de la Sociedad de Biología de Cuyo.; 2019

Sociedad de Biología de Cuyo

Tessaria absinthioides (Hook. & Arn.) DC, currently named ?pájaro bobo?, it is a frequent species inhabiting sandy and wet soils in Bolivia, Chile, Uruguay and Argentina. It is an aromatic plant commonly used in traditional medicine and as spices or seasonings through Argentina and Chile territories. Lately, our laboratory described the chemical composition of the leaves aqueous extract as well as its cancer-related properties. We demonstrated the cytotoxic activity against several cancer cell lines, discarded extract toxicity when is orally administrated to animals, and evidenced antitumoral effects against murine colorectal cancer. Despite of mentioned results, the molecular mechanism of action remains unclear. The goal of the present work is to compare the proliferation response of breast cancer cell lines to treatment with T. absinthioides aqueous extract (TaAE), paclitaxel (Ptx), carboplatin (Cbp) and doxorubicin (Dxr). Cell lines were selected by its molecular characteristics: MCF-7 (ER+, PR+, HER2-, p53wt), T47D (ER+, PR+, HER2-, p53mut) and MDA-MB-231 (ER-, PR-, HER2-, p53mut); and proliferation was studied by MTT assay after 48 h treatment. Statistics values of treatment potency were calculated with CompuSyn software. The treatment with TaAE resulted effective in all the cell lines studied: MCF-7 (Dm = 9.27 µg/ml; Fa 75 = 4.20 µg/ml; Fa 90 = 1.90 µg/ml); T47D (Dm = 25.12 µg/ml; Fa 75 = 17.89 µg/ml; Fa 90 = 12.74 µg/ml); MDA-MB-231 (Dm = 16.06 µg/ml; Fa 75 = 11.09 µg/ml; Fa 90 = 7.65 µg/ml). The obtained potency values confirm that TaAE activity against breast cancer cells it is not affected by ER, PR, HER2 nor p53 status. The results calculated in the treatments with commercial chemotherapics were highly variable between the different cell lines; the range of Dm were: Ptx = 0.218 ? 1.52 µg/ml; Cbp = 271.5 ? 840.08; Dxr = 0.12 ? 1.03. It is important to mention that T47D cells were not properly responsive to 48 h treatment with chemotherapics, while TaAE determine an evident cytotoxicity. Altogether, results support the study of TaAE as plant derived anticancer compound and open a new window to investigate the aqueous extract application as complementary agent in breast cancer treatment.