Prenatal D-Amphetamine exposure influences prolactin synthesis and secretion in response to stress and estrogen in adulthood

NEIRA FLAVIA J; BREGONZIO CLAUDIA; PIETROBÓN ELISA O; VALDEZ, SUSANA RUTH; SANTONJA FLORENCIA ELEONOTA; JAHN GRACIELA A

Mar del Plata

Congreso; Reunión conjunta de Sociedades de Biociencia; 2019

SAIC

The dopaminergic system, a main regulator of prolactin (PRL) secretion, is closely involved in the responses to stress and amphetamine. The ovarian steroids modulate PRL secretion and several aspects of stress responses. Previously, we found that prenatal exposure to amphetamine (PEA) induced changes in pituitary mRNA D2R expression during adulthood. Our present aim was to study PEA effects and the influence of estrogen (E2) on pituitary D2R expression (protein) and their correlation with pituitary PRL content (mRNA and protein levels) in adult OVX rats in response to stress. Moreover, we also explored the role of E2 in response to stress on the expression of de pTH-Ser 40 in the medium basal hypothalamus (MBH) of OVX adult rats in PEA animals. For these purposes, female Wistar rats were treated daily with D-amphetamine 2.5 mg/kg i.p./saline during days 15 to 21 of pregnancy. Their female offspring were OVX at day 60; 15 days later treated with estrogen/oil (E2; 2 x 5 µg/rat/24 h) and exposed to immobilization stress during 30 min. Blood and tissue samples were collected for corticosterone by RIA and pituitary D2R and PRL content by real time PCR and Western blot (WB) determinations. pTHSer-40 expression was determined by WB in MBH extracts. Data were analyzed using two-way ANOVA and Student´s-t test. Pituitary D2R expression (protein) was diminished only in PEA OVX+E2 rats (p