IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
LONG-TERM EFFECTS OF NEONATAL HYPOXIA ON ANXIETY-RELATED BEHAVIORS AND HORMONAL RESPONSE TO ACUTE STRESS
Autor/es:
PENNACCHIO GISELA E; SELTZER, ALICIA M; TORRECILLA, N M; JAHN, GRACIELA A; NEIRA FLAVIA J; SOAJE, MARTA; VALDEZ, SUSANA RUTH
Lugar:
San Luis
Reunión:
Congreso; Reunión Anual de la Sociedad de Biología de Cuyo; 2019
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Trabajo A 46Neonatal lesions in the brain have less severe effects than in adults due to the neuronal plasticity of the developing nervous system, although they can cause cognitive and behavioral sequelae. Previously, we found that neonatal hypoxia (NH) transiently affected the expression of proteins associated with synaptogenesis in certain brain areas. The intermingled neural circuits controlling both stress and anxiety suggest a strong relationship between stress experiences and anxiety in both healthy and pathological conditions. We evaluated the long-term effects of NH on anxiety parameters and in stress-induced hormone release in adult female (estrous day of rat cycle) and male rats. Sprague Dawley rats at 4 Post-Natal Day (PND) were exposed to an atmosphere of low oxygen level (6.5% O2 and 93.5% N2) for 70 min. 4PND control pups were exposed to normal oxygen levels (Co) for 70 min. The humidity and temperature conditions were controlled. Pups were then returned with their mother until weaned, and then they were allowed to grow. At 3 months of age, both groups of rats were subjected to two tests, Elevated Plus Maze (EPM) to measure anxiety parameters and a stressful challenge to determine hormone response to acute stress (exposure to ether vapors for 2 min). EPM reflected an unconditional aversion to heights and open spaces, an anxiogenic behavior. The hormonal response to stress included the release of pituitary prolactin (PRL), adrenal progesterone (P4), and adrenal corticosterone (CORT). Blood samples were collected before and after 5 min of stress exposure for serum hormone determinations by RIA. In the EPM test, both female and male hypoxic rats increased the number of entries to the open arms (OA) and the time spent in the OA compared to Co (P