Indole-3-carbinol reduces hypertension-induced cardiovascular remodeling and arrhytmias

CASAROTTO M.; BERETTA V.; DIEZ E.; PARRA M.; CUELLO CARRIÓN, F. D.; PONCE ZUMINO A. Z.; CALVO J. P.; CAMARGO A.; MANUCHA W.

Mendoza

Simposio; II Simposio Internacional de Medicina Traslacional; 2019

Facultad de Ciencias Médicas. Universidad Nacional de Cuyo

Hypertension and arrhythmias stand out as main causes of morbidity and mortality. During hypertension, nitric oxide (NO) and heat shock protein 70 (Hsp70) contribute to arrhythmogenic cardiac remodeling. A diet with low phytochemical content could worsen hypertensive remodeling through inflammation and oxidative stress modulation. Functional foods claim to reduce inflammation and oxidative stress by its high content of active phytochemicals. The indole-3-carbinol, present in cruciferous vegetables (broccoli and cabbages), protects against inflammatory response triggered by ischemia-reperfusion. Aim: To evaluate the effects of chronic treatment with indole-3-carbinol in hypertensive and normotensive rats summited to acute myocardial ischemia-reperfusion. Methods: Male SHR and WKY rats received indole-3-carbinol, or not (2000 ppm/day, per os) from birth to 8 weeks of age (n=10, each group). We assessed blood pressure and collected blood samples to evaluate biochemical/inflammatory markers. Per group, five cardiac samples we preserved for histology and molecular assays and five hearts underwent the following ischemia-reperfusion protocol: 10 min of pre-ischemia, 10 min regional ischemia and 10 min reperfusion. We evaluated action potentials, electrocardiogram and arrhythmias (regularity of rhythm, presence, and severity). Results: SHR rats exhibited histological, structural and functional changes with increased systolic blood pressure and inflammatory-oxidative markers (IL-6, TNF-α, NADPH activity, NO/iNOS/Hsp70, p