BENEFICIAL EFFECTS OF AQUEOUS EXTRACT OF TESSARIA ABSINTHIOIDES AND PROSOPIS STROMBULIFERA ON HUMAN ENDOTHELIAL VASCULAR CELLS

DE PAOLA, MATILDE; CASTRO, CLAUDIA; HAPON, MARÍA BELÉN; QUESADA, ISABEL; GAMARRA-LUQUES, CARLOS

Mendoza

Simposio; II Simposio Internacional de Medicina Traslacional; 2019

UNCuyo.

The use of herbals in the treatment of disease is a well established practice in traditional medicine. Tessaria absinthioides (Ta) and Prosopis strombulifera (Ps) are native plants from South-America with reported ethnopharmacological uses. Despite recent scientific reports about plant derived compounds identification and activities, there is not a well conducted research about its effects on cardiovascular disease (CAD). The aim of this work was to analyze the effects of aqueous extracts from leaves of Ta and Ps on human endothelial cells (HUVEC) isolated from human umbilical cord. Methods: After cell exposure to different concentrations of Ta and Ps intracellular reactive oxygen species (ROS) generation was measured by lucigenin-enhanced chemiluminescence and dihydroethidium assays. Total antioxidant capacity of treated cells was measured by TAS assay. RT-PCR, was utilized to examine NADPH oxidases (NOX) subunits expression. Results: Ta and Ps extracts (1 and 4 μg/ml) significantly increased the total antioxidant capacity of HUVEC cells (Ta in a dose-dependent manner). In the same way, Angiotensin II-induced HUVEC intracellular ROS generation was significantly diminished by the addition of 1 and 4 μg/ml of Ta or Ps extracts. Ps (4 μg/ml) was able to downregulate NOX2 (p< 0,05) and NOX5 (p