EFFECT OF TESSARIA ABSINTHIOIDES ON INTESTINAL INFLAMMATION AND IMPAIRED PERMEABILITY INDUCED BY LPS EX VIVO.

MAYRA VILCA; CLAUDIA CASTRO; MATILDE DE PAOLA; QUESADA, ISABEL; CARLOS GAMARRA-LUQUES

Mendoza

Jornada; V JORANDAS ACES Y JORNADAS DE HIPERTENSIÓN ARTERIAL DE CUYO; 2019

UNCuyo

ntestinal dysfunction consists of a defective barrier function, which allows the influx of luminal endotoxins, thus causing intestinal inflammation. Specifically, intestinal dysfunction has been associated with pathologies related to metabolism and/or diet, especially obesity. Based on the antioxidant and anti-inflammatory effects of Tessaria absinthioides (Ta) previously demonstrated in vivo and in vitro, we hypothesize that Ta consumption influences on intestinal health, protecting against inflammation and intestinal oxidative stress as well as dysfunction of the intestinal barrier caused by lipopolysaccharide (LPS) in an ex vivo intestinal model. The aim of this work was to analyze the bioactivity of an extract of Ta on inflammation and in the intestinal impaired permeability induced by LPS in an ex vivo model of mouse intestinal explants. To study the preventive effects of intestinal dysfunction, a 30-minute pretreatment was performed with three different concentrations of Ta (1, 10 and 100 mg/mL), then intestinal damage was induced with 10 µg/mL of LPS for 1 hour. Gene expression of NOX1, NOX2 (oxidative stress producing enzymes) and MCP-1 (inflammatory molecule) was analyzed by qRT-PCR. Treatment with 10 µg / mL of LPS was not able to produce changes in the expression of both NOX2 and MCP-1. However, 10 and 100 mg/mL of Ta significantly decreased NOX2 expression (P