ALLOPREGNANOLONE ACUTE EFFECT ON THE RAT OVARY FUNCTIONALITY.

LACONI, MYRIAM; CERIONI, SUSANA; VEGA, ADRIANA; YUNES, ROBERTO; CABRERA RICARDO

Rosario

Congreso; XLI REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE FARMACOLOGÍA EXPERIMENTAL; 2009

SAFE

B6-101 ALLOPREGNANOLONE ACUTE EFFECT ON THE RAT OVARY FUNCTIONALITY. M. Laconi, S.Cerioni, A.Vega, R.Yunes and R. Cabrera. LINCE-IMBECU-CONICET), F.C.M¨¦dicas, U.N.Cuyo. F.C.Salud. U.Mendoza. Mendoza. Argentina. mlaconi@lab.cricyt.edu.ar Allopregnanolone (ALL) is one of the best characterized neurosteroids in the brain. Previously we demonstrated that ALL inhibited the apoptotic process in the rat corpora lutea and caused a decrease in the 3¦Â HSD enzyme activity in the medio basal hypothalamus with an increase of this activity in the ovary on rat estrous day . The aim of this work was to study if ALL icv could modify the ovulation rate, ovaric (Pg) levels and ovaric histology during the estrous cycle. On the proestrous morning, Sprague Dawley adults female rats were injected in the third ventricle with ALL 6¦ÌM or vehicle and sacrificed 24, 48 and 72 hs after that. The ovaric and serum Pg levels were measured by RIA. One ovary was collected to histological studies and the other to Pg measures. The results were analized by ANOVA and post hoc-test, vs vehicle. p<0.05 was considered significant. ALL induced an increase in ovaric Pg levels at 48 hs (p<0.001), without significant changes at 24 and 72 hs. An inhibition of the ovulation was observed 24 hs post ALL without modification in the others groups. No significant changes were observed in the ovaric histology at the times of this study. We concluded that ALL would have different central and peripheral modulatory mechanisms of action on the regulation of the physiological reproductive female function, suggesting genomic and non-genomic mechanism in concert.