PHARMACOLOGICAL ACTIONS OF ALLOPREGNANOLONE OVER THE OVARIAN PHYSIOPATHOLOGY WITH DIFFERENT EXPERIMENTAL MODELS

SANHUEZA, MARÍA DE LOS ÁNGELES; PELEGRINA, LAURA TATIANA; ANTONELLA R. R. CÁCERES; LACONI, MYRIAM RAQUEL

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

Allopregnanolone (ALLO) is a neurosteroid derived from Progesterone; its actions in the CNS are well known. ALLO modulates GABA neurotransmission with antidepressant, anxiolytic, anticonvulsant and anesthetic effects. ALLO levels fluctuate under stress and throughout the menstrual cycle, pregnancy and menopause. The objective of this work was to evaluate ALLO action over the female rat ovarian physiopathology, from the global effect on the CNS to the action on the granulose cells. We designed different experimental models in order to differentiate four levels of action. After a dose/response curve we found that ALLO 6 µM, a pharmacological dose, was capable of disrupting the hypothalamus-hypophysis-ovarian axis causing significant ovarian morpho-physiological changes (augmented follicular atresia, p