IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Red wine polyphenols synergistically inhibit angiotensin II-induced cell migration and reactive oxygen species production
Autor/es:
REDONDO,ANALIA; ESTRELLA, NATALIA; CASTRO CLAUDIA; CRUZADO MONTSERRAT
Lugar:
San Luis
Reunión:
Congreso; XXVII Reunión de la Sociedad de Biología de Cuyo; 2009
Institución organizadora:
Sociedad de Biologia de Cuyo
Resumen:
Red wine polyphenols synergistically inhibit angiotensin II-induced cell migration and reactive oxygen species production Redondo A, Estrella N, Castro C and Cruzado M, Program I+D SeCTyP, FCM, UNCuyo, IMBECU, CONICET. mcruzado@fcm.uncu.edu.ar Dietary polyphenols are associated with the prevention of hypertension. Angiotensin II (AII) is an important humoral factor associated with this disease. Reactive oxygen species (ROS) are involved in AII-induced vascular action. We studied the effect of antioxidants present in red wine, quercetin (Q) and catechin (C), on AII-induced cell migration and ROS production in vascular smooth muscle cells (VSMC) obtained from small arteries of spontaneously hypertensive rats (SHR). We determined cell migration by scrape-wound assay, and ROS production by incubation with a fluorescent probe (H2DCF-DA). Data (mean±SEM) were analyzed by ANOVA and post test of Bonferroni. Q (10, 20 and 30 µg/mL) inhibited AII (10-7M) induced VSMC migration after 24 h stimulation instead C had no effect at the used concentrations. However, coincubation of Q(5µg/mL) plus C (6µg/mL) significantly inhibited AII-induced migration (157%±15,8 vs. 31,7%±7,2 **P