Effect of organosulfur compound derived from garlic on the migration and proliferation of vascular smooth muscle cells from spontaneously hypertensive rats

CLAUDIA MAGDALENA CASTRO

Mendoza

Congreso; 1º International Congress of Nutrition and Food Technology,; 2009

Garlic has been used for prevention and treatment of hypertension, however the molecular mechanisms of garlic’s effects remain to be elucidated. In the present study, the mechanisms of the in vitro effect of organosulphur compounds derived from garlic on growth and migration of cultured aortic smooth muscle cell (AoSMC) isolated from spontaneously hypertensive rats (SHR) were investigated. Allyl methyl sulphide (AMS) and diallyl sulphide (DAS) inhibited angiotensin II (AngII)-stimulated cell cycle progression and migration. Neither cell viability assay nor annexin-V binding analysis revealed cytotoxic effects of both organosulphur compounds at the concentrations used. Instead, their inhibitory effects were associated with preventing cell cycle inhibitor p27Kip1 (p27) downregulation and reducing the extracellular signal-regulated kinase (ERK)1/2 activity. When we assessed the antioxidant activity of AMS and DAS we found that both organosulphur compounds inhibited angiotensin II-induced reactive oxygen species (ROS) generation. Our findings show that compounds derivated from garlic not only have an antioxidant effect but also exhibit a protective effect against arterial remodeling seen in hypertension.