Topical anti-inflammatory activity and cyclooxygenases inhibition of decoction from Baccharis grisebachii.

GAMARRA LUQUES C; LIMA B; FERESIN GE; TAPIA A

San Luis

Congreso; XXXVII Reunión Anual de la Sociedad de Biología de Cuyo.; 2019

Sociedad de Biología de Cuyo

In traditional medicine from San Juan (Argentine), Baccharis grisebachii is used to treat ulcers, burns, and skin sores. The isolation of the p-coumaric acid derivatives and the flavonoids pectolinaringenin, nevadensin, dimethoxy sudachitin, 5,7,4-trihydroxy-6-methoxyflavone, quercetin, 5,7,4-trihydroxy-6,3-dimethoxyflavone and 5,7,4-trihydroxy-6,8-dimethoxyflavone were reported. The aim of the current work was to evaluate, topical anti-inflammatory effect in BALB/c mice and cyclooxygenases (COX 1 and COX 2) inhibition. The decoction was prepared and lyophilized (DC) from 10 g of dry material plant (100 ml of water, ebullition 15 min), and yields w/w were calculated. In vitro, cyclooxygenases (COX 1 and COX 2) inhibition was assayed by the colorimetric inhibitor detection Kit No. 701050 of COX (Cayman Chemical). Arachidonic acid (AA)-induced mice ear oedema as models of acute inflammation. The DC show an inhibitory effect to COX 2 (87.26%) to 36 µg/ml, and the percentage of inhibition in COX-1 was lower. The DC of B. grisebachii did not present activity in the mice model tested at 3 mg/ear. The results indicate that B. grisebachii possess a potential anti-inflammatory effect, by blocking the COX-2 proinflammatory enzyme pathway. Some constituents identified previously from B. grisebachii have been reported as anti-inflammatory; it is possible that these are responsible for the effect shown. Moreover, another model needs to be tested to confirm the decoction in vivo activity.