Recovery of the WT1 renal phenotype and atherogenesis reduction: a novel protective mechanism by organosulfur compounds treatment during cardiovascular disease.

CAROLINA TORRES; LUCIANA MAZZEI; ALEJANDRA CAMARGO; MATILDE DE PAOLA; MIGUEL FORNÉS; CLAUDIA CASTRO; MARCOS ARLANDI; DARÍO CUELLO-CARRIÓN; WALTER MANUCHA

San Luis

Congreso; XXXVII Reunión científica anual de la sociedad de biología de cuyo; 2019

Sociedad de Biología de Cuyo

Atherosclerosis is one of the leading causes of death from cardiovascular disease (CVD). Of interest, alterations in the transcription factor WT1 precede the development of CVD. The organogenic process -regulated by WT1- is modulated by oxidative/inflammatory (OI) mediators, and in this sense, it has been suggested to implement functional foods carrying organosulfur compounds (AF-OSCs) as a preventive anti-oxidative/anti-inflammatory strategy in CVD. So we decided to evaluate in a model of atherosclerosis, the possible renal changes of WT1, its correlation with OI markers, the impact on the development of atherosclerotic plaques, as well as the protection by the use of certain AF-OSCs. We used female mice deficient in apolipoprotein E (ApoE-KO) (N=8 per group) who received: a-standard feed (A); b-standard food + sunflower oil (B); or c-standard food + oil mashed with crushed garlic (380 ppm 2-vinyldithiine, 30 ppm garlic and 40 ppm diallyl sulfide) (C). After 2 months of the food protocol, blood was taken to evaluate biochemical parameters, renal tissue for microscopy, ultrastructure, IHQ, PCR, OI markers and thoracic aortas to quantify atheroma plaques by staining with Oil-red O. Group A evidenced a lipid, histological and functional profile consistent with the atherogenic process of the ApoE-KO hypercholesterolemic model. However, mice in group C showed a decrease in triglyceridemia after 2 months of treatment compared to group B (36.9 mg% ± 6.3 vs. 60.8 mg% ± 5.9; p