Immune cells from human blood leukocytes and murine spleen cells express mRNA of the prolactin receptor short isoform 3 and 1b respectively without being affected by stress

MORENO SOSA, TAMARA; MACKERN OBERTI, JUAN P.

Mendoza

Simposio; Symposiumon Translational Medicine; 2019

UNCuyo

Autoimmune diseases are heterogeneous and difficult to treat pathologies. Immune cells can be modulated by several endocrine mediators such as adrenalin, cortisol and prolactin. In the case of prolactin, it is known that liver cells express several isoforms including a long isoform (PRL-RL) that signals via STAT5 and JAK molecules, and a short isoform (PRL-RS) that signals only via JAK molecule. Although, it is known that immunce cells express the PRL-RL, it is not known whether immune cells express short isoforms. The aim of this work was to evaluate whether murine and human immune cells (from healthy and lupus) express the prolactin receptor short and long isoforms. To this end, C57BL/6 mice were euthanazed, spleen was harvested. Blood samples from healthy donors were harvested and red blood cells were lyzed. RNA from murine spleen and human blood leukocytes were obtained following trizol reagent protocol instructions. Then cDNA were synthesized with the subsequently PCR amplification. PCR products were visualized by agarose gel electrophoresis. We found that both murine and human leukocytes express mRNA PRL-RL. In addition, we also found that both murine and human leukocytes express the mRNA PRL-RS. Furthermore, spleen cells and PBMCs from lupus mice and patients displayed higher expression of mRNA PRL-RL form suggesting lupus cells are more sensible to prolactin. In conclusion, these results demonstrated that immune cells express both prolactin receptor isoforms and lupus immune cells displayed a differential expression . To our knowledge this is the first report that showed human leukocytes express PRL-RS.