Preliminary study on prevalence of class 1 integrons and extended-spectrum β-lactamases (esbl) in clinical isolates from mendoza hospitals, and tune-up tools for surveillance of intrahospital infections.

NOLLY M; DAMIANI MT; GARAY C; SÁNCHEZ D; MARQUEZ F; LEIVA N

Mendoza

Simposio; II International Symposium on Translational Medicine. XIII Annual Meeting of Investigation at the Faculty of Medical Sciences. Mendoza 7 y 8 de marzo de 2019, Mendoza, Argentina.; 2019

INTRODUCTIONBacterias have acquired the ability to survive the adverse conditions present in hospital environments. Also, the inadequate used of antibiotics turns them multiresistant, assigning this topic a relevant value concerning antibiotic administration policies.Antibiotic resistance caused by dissemination of genetic elements related to Class 1 Integron, is of growing interest worldwide due to an increase in the number of multiresistant strains, in particular to carbapenems and/or third and fourth generation of cephalosporins. Preliminary studies conducted by our laboratory reveal that 7 of 21 strains possess this Integron. The prevalence rate coincides with those found in Brazil, the United States, and the European Union.Random amplified polymorphic DNA (RAPD) is a technique used as a molecular marker based on PCR. The RAPD markers are derived from a single short primer with an arbitrary sequence, which can bind to the DNA in different parts of the genome, allowing the observation of a characteristic multi-band pattern for each analyzed genome.This technique is simple and inexpensive, however, it must be standardized with controls that ensure reliable and reproducible results.