IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Preliminary study on prevalence of class I integrons and extended-spectrum β-lactamases (EBSL) in clinical isolates from Mendoza hospitals, and tune-up tools for surveillance of intrahospital infections
Autor/es:
NOLLY M; DAMIANI MT; NOLLY M; DAMIANI MT; LEIVA N; SANCHEZ D; LEIVA N; SANCHEZ D; MARQUEZ F; GARAY C; MARQUEZ F; GARAY C
Lugar:
Mendoza
Reunión:
Congreso; XXXVI Reunión científica anual. Sociedad de Biología de Cuyo; 2018
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
The dissemination of genes for resistance to antibiotics by genetic elements, especially those related to class 1 integrons, is a growing concern worldwide due to the substantial increase of strains resistant to multiple drugs, especially those resistant to carbapenems and/or third and fourth generation cephalosporins. Resistance cassettes dissemination studies is an extremely relevant issue that concerns antibiotic administration policies. In this work, we determined the occurrence of class 1 integrons and extended-spectrum beta-lactamases (ESBL) in clinical isolates from local hospitals, using PCR techniques. On the other hand, nosocomial infections are among the most common complications in intensive care units. Bacteria are capable of surviving the adverse conditions found at the hospital environment, and the use of antibiotic pressure turns them multi-resistant to various antimicrobials. Therefore, we were interested in acquiring adequate and flexible tools to identify possible reservoirs of strains that propagate clonal infections inside the hospital. For this aim, we tune-up the Random Amplification of Polymorphic DNA (RAPDs) in order to be able to quickly identify infection focus, making possible an efficient control outbreak of clonal infection within hospitals.