Intermittent adenosine administered at reperfusion did not protect against ventricular arrhythmias

DIEZ ER, LÓPEZ MC, PONCE ZUMINO AZ, MIATELLO RM.

San Luis

Congreso; XXVII Annual Scientific Meeting CUYO BIOLOGY SOCIETY; 2009

Sociedad de Biología de Cuyo

Postconditioning (PC) maneuvers as 3 cycles of 30-s reperfusion/ischemia or short-term adenosine treatment performed immediately after the 10 min ischemia reduced reperfusion arrhythmias (RA) in rat hearts. Here we tested whether intermittent adenosine (IADO) may trigger PC-like cardioprotection. Isolated rat hearts underwent 10 min regional ischemia and 15 min reperfusion. After ischemia, 3 cycles of 30-s reperfusion/adenosine 10 µM (IADO10) and 100 µM (IADO100) (both n=10) where compared with control (n=13). We analyzed the incidence and severity of RA and its relationship with changes on ECG and action potentials recorded simultaneously. Control group hearts developed RA in 11/13, IADO 10 µM in 8/10 and IADO 100 µM in 10/10 (p>0.05 by χ2). Sinus rhythm was restored in 2, 2 and 4 hearts in control, IADO10 and IADO100 respectively. IADO100 reduced action potential duration from 53.7 ± 5.2 ms to 36.6 ± 8.4 ms (p