IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ECGR2 AND b-CATENIN CATENIN ARE POTENTIAL BIOMARKERS TO EARLY DIAGNOSIS IN ORAL SQUAMOUS CELL CARCINOMA
Autor/es:
PENNACCHIOTTI, GINA; VALDÉZ, FABIO; RUBÉN W. CARÓN; GÓMEZ, SILVINA; EZQUER, MARCELO; FLAVIA A, BRUNA; FANELLI, MARIEL; FERNÁNDEZ-RAMIRES, RICARDO
Lugar:
Santiago
Reunión:
Congreso; VIII Jornadas de Patología Oral de Chile; 2018
Institución organizadora:
Universidad de Chile, Facultad de Odontología
Resumen:
The oral squamous cell carcinoma (OSCC) is the most common malignancy of the head and neck, with more than 300,000 new cases reported annually worldwide. The disease has a high morbidity rate (37.8%) five years after diagnosis; despite the progress in research and therapy, survival has not improved significantly in the last few decades. Several tumor biomarkers have been suggested to anticipate the OSCC prognosis related with cancer outcome with poor results. The aim of this study was to evaluate if ECGR2 and β-Catenin are good predictive biomarkers during the OSCC progression. Oral mucosal biopsies from Chilean patient?s with dysplasia or carcinoma (n=20) where collected. The mRNA was extracted for gene expression quantification of ECGR2, NF-kB, P53, RB1, β-Catenin and CYP4B1. Additionally the ECGR2 and β-Catenin were correlated with their protein presence by IHQ. One-Way ANOVA and Dunn?s post hoc test were used to data analyze. We found in OSCC group ECGR2, β-Catenin and P53 gene expression significantly decreased respect to normal subjects. Meanwhile RB1, Nf-KB and CYP4B1 were significantly increased in OSCC. The H&E analysis of dysplasia group showed an oral epithelium of 4 cell layers with moderate inflammation and absence of invasion. The OSCC group showed an epithelium of 8 cell layers, invasion of epithelial cells in the stroma and a severe inflammation. The ECGR2 protein in dysplasia group showed a scarce mark in the suprabasal layer, while the OSCC group was negative. Moreover β-Catenin was found located in the nucleus in OSCC biopsies correlating with a poor prognosis. Our results suggest that ECGR2 and β-Catenin can be used to predict OSCC progression. Both biomarkers have the potential to be applied as a possible early diagnostic method.