IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IGF-1 expression in the cerebellum of the developing Spontaneously Hypertensive Rat (SHR)
Autor/es:
ASENSIO JOANA ANTONELA; SELTZER ALICIA M; SUSANA R. VALDEZ; BENITEZ SERGIO
Lugar:
Córdoba
Reunión:
Congreso; XXXIII SAN; 2018
Institución organizadora:
Sociedad Argentina de Neurociencias
Resumen:
The Spontaneously Hypertensive Rat (SHR) grows in a chronic hypoxic environment due to placentalinsufficiency. This situation resembles that of the IUGR, one of the majorproblems in perinatal medicine representing one of main causes of perinatalmortality and morbidity. Insulin-like growth factor 1 (IGF-1) serves as apromoting factor for Purkinje cell postnatal survival and dendritic growth, andit stimulates repair mechanisms after hypoxic damage. An increase in IGF-1levels has been associated with enhanced reactive astrocytosis. The refinementof neuronal circuits during postnatal (P) cerebellar development is critical totheir subsequent function and abnormalities in this process can result inneurodevelopmental disorders, as shown by the SHR rats. We examined theexpression of IGF-1 by RT-PCR in the cerebellum of SHR and of the normotensivecounterparts of the WKY strain, at P7 and P14. We also measured GFAP immunofluorescencein the cerebellum white matter (WM) and c. callosum (CC) of littermates. Wefound an increase IGF-1 expression in the WM of the SHR at P14 (p<0.01 vsWKY). In a preliminary assay we detected GFAP immunolabeling in WM and CC at P7and P14 in both strains. This reactivity is apparently more intense in the SHRanimals. These results indicate that the SHR brain may show signs of brainrepair and remodelling as a consequence of an adverse gestational environment. SHRrat could be proposed as a valid animal model for studying IUGR. &amp;lt;!-- /* Font Definitions */@font-face{font-family:Arial;panose-1:2 11 6 4 2 2 2 2 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536859905 -1073711037 9 0 511 0;}@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1107305727 0 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:auto;mso-font-pitch:variable;mso-font-signature:-536870145 1073786111 1 0 415 0;} /* Style Definitions */p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin-top:0cm;margin-right:0cm;margin-bottom:8.0pt;margin-left:0cm;line-height:107%;mso-pagination:widow-orphan;font-size:11.0pt;font-family:Calibri;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:EN-GB;mso-fareast-language:EN-US;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-size:11.0pt;mso-ansi-font-size:11.0pt;mso-bidi-font-size:11.0pt;font-family:Calibri;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-ansi-language:ES-AR;mso-fareast-language:EN-US;}.MsoPapDefault{mso-style-type:export-only;margin-bottom:8.0pt;line-height:107%;}@page WordSection1{size:612.0pt 792.0pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}--&amp;gt;