IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Copy number variation of the Heat Shock Proteins in cancer
Autor/es:
FERNANDEZ-MUÑOZ, JUAN MANUEL; ZOPPINO FELIPE CARLOS MARTIN; CIOCCA, DANIEL RAMON; CIOCCA, DANIEL RAMON; GUERRERO GIMENEZ, MARTIN EDUARDO; GUERRERO GIMENEZ, MARTIN EDUARDO; FERNANDEZ-MUÑOZ, JUAN MANUEL; ZOPPINO FELIPE CARLOS MARTIN
Lugar:
Mendoza
Reunión:
Simposio; 24th Latin-American Symposium on biotechnology, biomedical, biopharmaceutical, and industrial applications of capillary electrophoresis and microchips technology; 2018
Institución organizadora:
LACE
Resumen:
The heat shock proteins (HSPs) induced by cell stress are expressed at high levels in a wide range of tumors and are closely associated with a poor prognosis and resistance to anticancer therapy.Copy number variations (CNVs) have a critical role in cancer development and progression. A chromosomal segment can be deleted or amplified as a result of genomic rearrangements, such as deletions, duplications, insertions, and translocations. CNVs are genomic regions greater than 1 kb with an alteration of copy number between two conditions (e.g., Tumor versus Normal). A CNV study of the entire HSP family in different cancers is lacking. The purpose of this work is the CNV analysis of HSP in breast, prostate and ovarian cancers and then perform a correlation analysis between the aberrations found and the expression levels of the HSP. The data were retrieved from the public available ?The Cancer Genome Atlas? (TCGA) database. The segmentation data provide for de CNV analysis used the Affymetrix Genome-Wide Human SNP Array 6.0 platform. Also, we have examined the levels of 50,000 genes in the 3 cancer types paying particular attention to the HSP genes. The RNA levels were retrieved from the TCGA using the omic-platform RNA-seq methodology for RNA profiling.The Bioconductor package CNtools was used to find the aberrations between the different tumor types.Our study revealed that 3 HSPs present amplifications in breast cancer (CCT3, DNAJC5B, ODF1) and no deletions were found. In Ovarian cancer 9 HSPs were found with amplifications (CCT3, CCT5, DNAJB1, DNAJB4, DNAJB8, DNAJB11, DNAJC13, DNAJC19 and DNAJ5B), and 6 HSPs with deletions (BBS12, CCT6B, DNAJB7, DNAJB14, DNAJC7 and DNAC15). No amplification or deletions were found in prostate cancer.