HYPOGLYCEMIC AND ANTIOXIDANT EFFECTS OF MULINUM SPINOSUM IN MICE WITH METABOLIC SYNDROME INDUCED BY HIGH FAT-FRUCTOSE DIET

BERRUEZO,S; CASTRO, C; SCAPINI, C; CREMER, C

Congreso; XXXVI Reunión Cientifica Anual De La Sociedad De Biología De Cuyo; 2018

SOCIEDAD DE BIOLOGÍA DE CUYO

Mulinum spinosum, popularly known as ?neneo?, has a broad distribution in the Patagonian steppe. Traditionally, an aromatic bitter decoction prepared from the root of this plant is used for different purposes. Due to the widespread utilization of the plant as a traditional medicament, the present work investigated the effects of its consumption in an animal model of Metabolic Syndrome (MS). Neneo roots were dried, crushed and used to prepare an infusion according to the traditional method. To generate the MS model, C57/BL6 adult male mice were fed with a 20% w/v fructose (Fru) in drinking water and a 30% w/w high fat diet (HFD) over a period of 5 weeks. After MS was confirmed by intraperitoneal glucose tolerance test (IPGTT), the neneo infusion was administered (8g/Kg/day) together with Fru-HFD during 12 weeks. At the end of the exposure period, mice were anesthetized and sacrificed by cardiac puncture to obtain blood samples. The Fru-HFD group significantly increased body weight, total serum cholesterol, HDL-col, LDL-col and serum lipid peroxidation (MDA) compared to control group. The treatment with neneo during 12 weeks, decreased glycemia and MDA compared to Fru-HFD group (p