IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
The human adipose tissue secrete components that regulate the tumor and non tumor renal epithelial cells behavior
Autor/es:
ROMEO L; SASSO CV; CARÓN RW; BRUNA F; SANTIANO F; LÓPEZ FONTANA C; GOMEZ SE; ZYLA L; PISTONE CREYDT V
Lugar:
Mar del Plata
Reunión:
Congreso; LXIII Reunión Científica de la Sociedad Argentina de Investigación Clínica; 2018
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
An essential information exchange is established between renal epithelial cells and adipose/fibroblastic stroma. In the present work, we evaluated the conditioned media (CMs) effects of human adipose tissue explants from normal (hRAN) and tumor (hRAT) kidney on: proliferation, adhesion and migration of tumor (786-O, ACHN, Caki-1) and non tumor (HK-2) renal epithelial cells. We also evaluated pERK/ERK y pPI3K/PI3K changes in different cell lines incubated by 2 and 24 h with hRAN-, hRAT, or control-CMs. Human renal adipose tissues were obtained from patients with renal cell carcinoma (hRAT, n=9) and kidney donors (hRAN, n=10). The CMs of hRAN and hRAT were collected 24 h post incubation and cells were treated with CMs. Proliferation (MTT assay), adhesion and migration (wound-healing and transwells assay) were evaluated in 786-O, ACHN, Caki-1 and HK-2 cell lines incubated with different CMs. The expression of pERK/ERK and pPI3K/PI3K on cell lines incubated with CMs (WB assay). Statistical differences among experimental conditions were evaluated by one-way ANOVA with Tukey´s post hoc tests.All cell lines showed a significant decrease in cell adhesion (p