Grape pomace extract reduced reperfusion arrhythmias in rats with a high-fat-fructose diet. .

ANTONIOLLI A; RODRIGUEZ LANZI MC; MIATELLO RM; BOTTINI R; VAZQUEZ PRIETO MA; PERDICARO DJ; FONTANA AF; DIEZ ER

Mendoza

Congreso; Interamerican Society of Hypertension. XXIV Congreso Argentino de Hipertensión Arterial; 2017

Sociedad Argentina de Hipertensión Arterial

Metabolic syndrome (MetS) is a risk factor of sudden cardiac death. Grape pomace (GP), obtained from winemaking process, contains phenolic compounds with potential cardioprotective effects. The aim of this study was to evaluate if a high-fructose-fat (HFF) diet facilitates the occurrence of arrhythmias during the reperfusion, and if a GP supplementation could counteracts this effects. Eight weeks male Wistar rats were randomly assigned for 6-week to the following 3 groups (8 rats per group): Control diet (C), High Fat-Fructose diet (HFF) (20 % bovine fat and 20 % fructose w/w added to the standard chow), and HFF supplemented with Grape pomace (HFF+GP) in a dose of 1g Kg-1day-1. HFF diet induces characteristic features of MetS (higher systolic blood pleasure, dyslipidemia and insulin resistance) which were attenuated by GP supplementation. In addition, HFF induced increased heart weight and reperfusion arrhythmias that were reduced upon GP supplementation. Moreover, GP improves heart p-AKT and p-eNOS expression and reduces Nox4 expression enhanced by a HFF diet, indicating that GP may increase NO? bioavailability in heart. GP counteracts diet-induced heart dysfunction and metabolic alterations. The potential of GP as source of natural bioactive compounds could revalorize this byproduct of winemaking process.