STUDY OF THE BROWNING PROCESS OF BREAST CANCER ADIPOSE TISSUE

FLETCHER S; CROSBIE ML; SANCHEZ, MELISA; AMATO, AR; DRESZMAN, R; TONEATTO, J; URSINO, ANABELA; CALVO, JC; PISTONE CREYDT, VIRGINIA; SANTISO, NATALIA; GUTIERREZ, A

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Abstract: Adipose microenvironment is involved in signaling pathwaysthat influence breast cancer progression. Although adipocyteshave been shown to promote breast cancer development,adipocyte characteristics involved in this process remain poorlyunderstood. The aim of this work was to investigate the effects offactors derived from conditioned media (CMs) from human breastcancer adipose tissue explants (hATT) or normal breast adipose tissueexplants (hATN) on induction of white adipocyte cell line browning.Morphology changes and brown adipose tissue (BAT)-relatedmarkers (UCP1, PRDM16, PGC1α and TBX1 among others) expressionwere evaluated following exposure of 3T3-L1 adipocytesto hATT or hATN CM. Increased expression of UCP1, PRDM16 andPGC1α was observed in adipocytes 3T3-L1 exposed to hATT CM incomparison to hATN CM. Interestingly, adipocytes exposed to hATTCM displayed characteristics that morphologically resembled brownadipocytes. In contrast, adipocytes exposed to hATN CM increasedlipid droplet size, characteristic features of white adipocytes. Insummary, these findings suggest that hATT secrete a different setof factors compared to hATN, which may induce browning of whiteadipocytes. This simple experimental approach suggests that hATTattached to the tumor could induce white adipocyte browning presentin its microenvironment, for paracrine signaling.