IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Desmoglein-4 deficiency increases resident CD4+ T cell subset in skin
Autor/es:
NEIRA, FLAVIA; VALDEZ, SUSAN RUTH; MORENO-SOSA, TAMARA; PIETROBON, ELISA; PENNACHIO, GISELA; SOAJE, MARTA; JAHN, GRACIELA ALMA; MACKERN OBERTI, JUAN PABLO
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión conjunta de las sociedades biomedicas 2017; 2017
Institución organizadora:
Reunión Conjunta de Sociedades de Biociencias
Resumen:
It is known that desmogleins are involved in cell to cell adhesion mechanisms. Desmogleins linked to intracellular and extracellular molecules are crucial in keeping structural and architecture integrity of different tissues including skin, nervous system and gut. These type of molecules, such as desmoglein-3 plays important roles in differentiation, cell activation and migration acting as regulators in the control of key molecules and signaling events such as actin and MAPK pathway. In addition, it is known that keratinocytes produce several inflammatory factors such as IL17 and KC that modulate T cells and leukocytes. However, whether desmoglein-4 present in keratinocytes may drive inhibitory or activating signals to skin-resident immune cells is absolutely unknown. The role of desmoglein-4 in keeping immune homeostasis in the skin has not been assessed which be a therapeutic target in skin cancer, psoriasis and dermatitis. The aim of our work was to assess the impact of desmoglein-4 deficiency in the amount of skin leukocyte population. To this end, OFA hr/hr rats which are mutant for the desmoglein-4 gene and Sprague-Dawley (SD) wild type rats were used. Skin biopsies from OFA and SD rats were weighted, minced to obtained cell suspensions and stained with monoclonal antibodies against CD45 (panleukocytary lineage cell marker) and CD3 ( T cell lineage cell marker) conjugated with APC and FITC respectively. Beads were added to stained cell suspensions from skin biopsies derived from OFA and SD rats and acquired by a FACS to measure the presence of total counts of cells per milligram of tissue. Interestingly, we found that OFA rats showed an expansion of CD45+ cells compared to SD control rats (%± vs ±) (p