Anterior pituitary-nitric oxide production is modulated by spleen macrophage secretions in rat with polycystic ovary

GISELA MENDOZA; MYRIAM FORNERIS; LUCIANA MAZZEI; WALTER MANUCHA

Merlo

Jornada; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2017

Sociedad de Biología de Cuyo

Polycystic ovarian syndrome (PCO) is one of the most common endocrinopathies among women of reproductive age. Previously, we reported a functional relationship between neuroendocrine and immune systems in a rat model of PCO, and it was observed that macrophage secretions (Mφ-S) in anterior pituitary gland (AP) modified the gonadotropins release and apoptosis. Nitric oxide (NO) and Heat Shock Protein70 (Hsp70) have been related to PCO pathogenesis. In rats, NO participates in the regulation of proliferation/apoptosis of pituitary cells and induce an increase of Hsp70 expression, but is not known the interaction of those markers with immune cells on AP in PCO. In this work, was studied whether PCO-Mφ-S affect the NO release and, iNOS and Hsp70 mRNA and protein expression from AP of PCO and Control (C) rats. PCO condition was induced in adult rats by estradiol valerate (2 mg/rat), and after two months the rats were sacrificed. Spleen Mφ from PCO rats were cultured (1x106 cells) for 24h in RPMI medium. Their secretions were used to stimulate PCO-AP and C-AP for 3h in metabolic bath. The NO release was measured as nitrites by Griess reaction and iNOS and Hsp70 expression were assayed by RT-PCR and Western Blot. The PCO-Mφ-S in relation to respective basal: increased the NO release from PCO-AP (p