ADIPOSE TISSUE OF HYPERTHYROID RATS REDUCES SURVIVAL AND MIGRATION OF MAMMARY TUMOR CELLS

CALLEGARI E; CARÓN RW; ÁVILA MANIERO M; BRUNA FLAVIA; ZYLA LE; SANTIANO F; LÓPEZ FONTANA C; CALLEGARI E; CARÓN RW; PISTONE CREYDT V; ÁVILA MANIERO M; BRUNA FLAVIA; SASSO CV; ZYLA LE; SANTIANO F; LÓPEZ FONTANA C; PISTONE CREYDT V; SASSO CV

San Luis

Congreso; XXXV Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2017

SOCIEDAD DE BIOLOGIA DE CUYO

We evaluated changes in the viability, adhesion and migration in ex vivo cultures of mammary tumors and in tumoral and non-tumoral mammary epithelial cell lines incubated with conditioned media (CMs) from mammary adipose tissue explants (TAM) of hyperthyroid (Hyper) and euthyroid rats (EUT). Female Sprague-Dawley rats were treated with dimethylbenzathracene (15 mg/rat) at 55 days of age and divided into two groups: Hyper (T4, 0.25 mg/ml/kg injected s.c. daily, n=10) and EUT (untreated control, n=10). At sacrifice, TAM was obtained and the CMs were collected after 24 h of incubation with M199. Ex vivo cultures of mammary tumors from EUT, MCF-10A, MCF-7 and MDA-MB-231 cells were grown and incubated with TAM-CMs of Hyper or EUT rats. Viability, adhesion and migration ability of cells were quantified. Soluble components present in CMs were identified by proteomics. Proteins were separated in polyacrylamide gels. The identification of proteins from complex samples was performed by two-dimensional nano-liquid chromatography (2D-nanoLC)-mass spectrometry (MS/MS) and analyzed with ProteoIQ (Premier Biosoft) software. Ex vivo cultures and tumor and non-tumor cell lines incubated with MCs-TAM-Hyper survived and migrated significantly less than those incubated with MCs-TAM-EUT (p