IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Broad protective efficacy of a universal influenza vaccine based on nucleoprotein adjuvanted with the c-di-AMP administered subcutaneously
Autor/es:
SCODELLER, EDUARDO ALBERTO; EBENSEN, THOMAS; GUZMAN, CARLOS ALBERTO; SCHULZE, KAI; CARGNELUTTI, DIEGO ESTEBAN; SANCHEZ, MARIA VICTORIA
Lugar:
Sao Paulo
Reunión:
Otro; IUIS Brazil Advanced Course on Vaccine; 2017
Institución organizadora:
Bill and Melinda Gates Foundation, USP-Universidad de Sao Paulo, International Union of Immunological Societies
Resumen:
Background: Influenza is a respiratory disease with a high annual global impact in terms of morbidity and mortality. Influenza vaccines are based on the induction of antibodies which are not fully capable of protecting against new antigenic variants. The formulation of vaccines including conserved viral antigens and novel adjuvants capable of inducing strong cellular responses, could be a valuable strategy for the development of a universal vaccine. Objective: In this study, we evaluated the efficacy of a vaccine formulated with the recombinant viral nucleoprotein (NPr) in combination with the STING agonist, c-di-AMP, to protect against influenza. Methods and results: Groups of BALB / c mice were immunized on days 1 and 21 with saline solution (control), NPr alone, and NPr + c-di-AMP, by subcutaneous route. At day 42, humoral and cellular immune responses were evaluated. The adjuvanted formulation induced high titers of IgG antigen specific antibodies IgG (4x105) and IgG1 (8.9x105) and IgG2a (4.8x105) subtypes, compared to immunization with NPr alone with IgG titers (8x105), IgG1 (3.6x105) and IgG2a (1.84x104). The c-di-AMP formulation induced a Th1