IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EFFECT OF GRAPE POMACE EXTRACT RICH IN POLYPHENOLS ON ADIPOSE TISSUE ANGIOGENESIS AND INFLAMMATION IN RATS WITH HIGH-FAT DIET
Autor/es:
PERDICARO DJ; PICCOLI P; FONTANA AF; VAZQUEZ PRIETO MA; RODRIGUEZ LANZI MC; MIATELLO RM
Lugar:
BUENOS AIRES
Reunión:
Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017
Resumen:
Adipogenesis and angiogenesis may counteract epididymal adipose tissue (eWAT) dysfunction under exposure to high-fat diet (HFD). Polyphenols has been found to exert anti-inflammatory effecs on eWAT. The aim of this study was to evaluate the effect of grape pomace extract (GPE) on eWAT angiogenesis/adipogenesis and inflammation from Spontaneously Hypertensive (SHR) and Wistar-Kyoto (WKY) rats receiving a HFD. SHR/WKY rats were divided: Control group SHR/WKY; SHR/WKY-HFD group receiving fat (40% (w/w)); and SHR/WKY- HFD supplemented with GPE (300 mg/Kg/d) for 10 weeks. HFD consumption led to increased body weight in WKY rats compared to both Ctrl and SHR strain. HFD significantly increased eWAT weight and adipocyte size in WKY rats, suggestive of hypertrophy. WKY rats supplemented with GPE had smaller adipocytes without changes in adipose weight, suggestive of hyperplasia. HFD did not affect eWAT weight and the diameter of adipocytes in SHR rats. GPE supplementation led to higher eWAT PPARg protein levels in both SHR and WKY rats and higher mRNA levels in SHR compared to WKY/SHR HF and Ctrl groups. In HF rats the protein levels of vascular endothelial growth factor A (VEGF-A) increased in both strains, while VEGF-R2 were higher only in SHR rats. In addition, GPE supplementation enhanced VEGF-A and VEGF-R2 protein levels in WKY compared to WKY HF and Ctrl rats. In contrast, the VEGF-A mRNA expression was enhanced by GPE in SHR. HFD caused higher levels of TNFα and MCP-1 mRNA, and CD68 protein in SHR rats, which was prevented by GPE supplementation. Protein levels of resistin, were higher in WKY HF and in SHR groups, while those of Nox4 were higher in the WKY/SHR HF groups. GPE supplementation significantly reduced HF-induced increase of resistin and Nox4 protein in both strains. Considering these results, supplementation with food rich in bioactive compounds can attenuate eWAT inflammation perhaps by its effect on increase eWAT angiogenesis and adipogenesis.