Regulation and action of thyroid hormones during early pregnancy in rats

CAMPO VERDE ARBOCCO F; GAMARRA-LUQUES C; RINALDINI E; HAPON MB; PENA V

Mar del Plata, Buenos Aires

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2016

One limiting processes of reproductive success is implantation.During this period steroid hormones (E2 and P4) stimulatethe synthesis of vascular endothelial growth factor (VEGF-A), themain modulator of angiogenesis during peri-implantation period.Our recent data show that hypothyroidism affects the process ofimplantation in the rat. Therefore, the goal of this investigation wasto elucidate the mechanisms by which thyroid hormones (THs)act at uterine level during implantation. For this purpose, wereused adult female rats (Wistar). Hypothyroidism was induced byadministration of propylthiouracil in the drinking water. Factorsrelated to the regulation of THs transport into the cell interior,intracellular metabolism and differential expression of their receptorswere determined by RT-qPCR. The interaction betweenTHs and the mechanism of action of E2, P4 and angiogenesis,were studied during implantation in utero by Western blot. Theresults of this study indicated that the mRNA expression of thedeiodinase enzyme 2 and thyroid hormone receptors (Thra andThrb) decreased significantly (p