Vitamin A deficiency alters hormone receptors and morphology´s virgin mammary gland

CAMPO VERDE ARBOCCO F; JAHN GA; OROZCO AL; MOHAMED F; VASQUEZ GOMEZ ME; FILLIPA V; GIMENEZ MS

Mar del Plata

Congreso; LXI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2016

Sociedad Argentina de Investigación Clínica

Vitamin A (VA) is important to growth of epithelial tissues and promotes the formation of lumen by mammary epithelial cells in vitro and suggests that plays a similar role in vivo. The effect of vitamin A deficiency (VAD) in the expression of hormone receptor, morphology and proliferation were determined. Wistar female virgin rats were separated into 6 groups: fed with VA diet for 3 months (c3m), VAD diet for 3 months (d3m), VAD diet for 75 days followed by refeeding with VA for 15 days (r3m), VA diet for 6 months (c6m), VAD diet for 6 months (d6m) and VAD diet for 150 days followed by refeeding with VA for 30 days (r6m). The expressions of estrogen receptors (REα and REα), prolactin receptor (RPrl), progesterone receptor B (RPB) were measured by RT-qPCR, fosfocolin citidililtranferase (CTα) by RT-PCR; fatty acids (FA) and unsaturation index of phospholipids (UIP) by FAME; PCNA by immunohistochemistry and morphology by hematoxylineosin. The expression of REα increased whereas REα and RPB decreased in d6m respect to c6m and r6m (p