PROGESTERONE AND ESTROGEN PREVENTS THE APPEARANCE OF PREMOTOR AND MOTOR SIGNS IN MALE HEMIPARKINSONIAN RATS

NATALIA BELÉN GONZALEZ; SEBASTIAN GARCÍA; MARIA BELEN MULLE BERNEDO; ANTONELLA ROSARIO RAMONA CÁCERES GIMENEZ; RIACARDO CABRERA

Mar del plata, Buenos Aires

Congreso; Reunión Conjunta 2016 entre la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Farmacología Experimental (SAFE); 2016

Sociedad Argentina de Farmacología Experimental (SAFE)

Parkinsons diseaseis the second most prevalent neurodegenerative disease after Alzheimer. A 6-hydroxydopamine(6-OHDA) lesion simulates the motor deficits that occur due to a depletion  of dopamine neurons in the nigrostriatal pathway,and has the advantageof presenting side-biased motor impairment. Some reports concerning motordeficits indicate a favourable response to steroid administration in hemiparkinsoniananimals. The objective was to evaluate the neuroprotection of theneuroactivesteroids, progesteroneand estrogen, over motor and pre-motor signs induced  by a 6-OHDA lesion. Sprague-dawley male rats (280-320g)60 days old were used. Hemiparkinsonism was induced by 6-OHDA stereotaxiclesion in the left corpus striatum. The experimental groups were: Sham (S),hemiparkinsonian (HP), and hemiparkinsonian treatedsubcutaneously with estrogen and progesterone (HPEP). Premotor signs wereevaluated by a force swimming test and motor signs by raturn. Data wereanalysed by ANOVA-1 and  Tukey?s post hoc.A significance decrease (p<0.01) was observed in the time spend swimming onthe force swimming test between S and HP groups. While there were nosignificant differences in S vs HPEP. The injection of 6-OHDA induced asignificant change in the turning  behaviourto the left side in HP (p<0.01) and HPEP (p<0.05) groups vs the S group.We conclude that the estrogen and progesterone treatment prevents the onset ofdepression like behaviour in HP rats and reduces the motor signs induced by a 6-OHDAlesion. Estrogen and progesterone exerts a neuroprotective effect against Parkinsondisease.