IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Genomic effects of the alumina nanoinsecticide ?NSA? in human peripheral blood lymphocytes
Autor/es:
VALDEZ, S.; STADLER, T.; NADIN, S. B.
Lugar:
Mendoza
Reunión:
Simposio; XXXIV REUNIÓN CIENTIFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO ?ASPECTOS BÁSICOS- TRASLACIONALES EN CIENCIAS BIOLÓGICAS?; 2016
Institución organizadora:
Facultad de Ciencias Médicas, U.N.Cuyo
Resumen:
In the last years, there was increasing interest to explore natural chemical pesticides in agriculture. The inorganic alumina nanoinsecticide "NSA" is the ideal candidate of biopesticide because of its high efficiency and reduced possibilities to induce resistance. In spite of the numerous studies on the toxicity of nanoparticles in in vitro systems, however, it remains to determine NSA risks for human health and the environment. Objectives: To determine whether NSA induces DNA damage in human peripheral blood mononuclear cells, and to compare with natural products and pesticides. Methodology: Peripheral blood lymphocytes (PBL) were isolated from a healthy donor venous blood and cultured at 37°C in RPMI 1640 medium supplemented with: 10% fetal bovine serum, 5 μg/mL phytohemagglutinin, 2 mM L-glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin. PBL were exposed for 24 hours to increasing concentrations of NSA, Al2O3 and SiO2 (50, 100 and 200 μg/ml) and then collected. To study DNA damage and chromosomal damage we used alkaline comet assay and micronuclei (MIN) test, respectively. Cell viability was tested with resazurin assay. Results: Comet assay revealed no significant increase of DNA damage by NSA compared with other natural substances. As expected, DNA breaks were significantly higher in cells exposed to organophosphorades, OPS (P