ADIPOSE TISSUE AND EPITHELIAL CELL: A DANGEROUS DYNAMIC DUO IN BREAST AND KIDNEY CANCER

PISTONE CREYDT, VIRGINIA

Mendoza

Congreso; XXXIV Reunión Anual de la Sociedad de Biología de Cuyo; 2016

Normal morphogeneis andfunctionality, as well as cancer development, require essential information exchangebetween epithelial tissue and fibroblastic/adipose stroma. Adipose tissue (AT)is a bioactive endocrine organ that secretes soluble factors and contributessignificantly to the composition of the extracellular matrix. Adiposemicroenvironment is involved in signaling pathways that influence breast andkidney cancer. We showed that conditioned media (CMs) of human adipose tissuefrom tumor breast (hATT) differentially regulate proliferation, adhesion andmigration of breast cancer epithelial cell lines (MCF-7 and IBH-7), as opposedto hATN-CMs (human adipose tissue from normal breast-CMs). Therefore, ourresults show that proteoglycan versican, membrane protein HCAM and adiponectinreceptor type 1 (AdipoR1) are possibly involved in the biological effectsobserved. Additionally, demonstrated that CMs from human AT explants from renalcell carcinoma near the tumor (hRATnT) regulate adhesion and migration of tumor(786-O, ACHN) and non tumor (NK-2) renal epithelialcells, contrary to CMs from human AT explants from renal cell carcinoma fartheraway from the tumor (hRATfT). We observeda higher expression of versican, leptin and adiponectin, as well as a lowerexpression of ADAMTS1 in hRATnT-CMs compared to the expression in hRATfT-CM. We developed a model in which we obtained CMs from ATexplants completely, from both normal and tumor breast or kidney. Also, weshowed hypothyroidism produces changes in the ability of AT to secrete solublefactors that regulate the survival and migration of tumor and non-tumor mammarycells. The identification of these factors, both in AT and epithelial cells mighthelp develop new strategies to prevent and/or treat cancer.