Perivascular adipose tissue secretome participates in the development of hypertension

REDONDO ANALIA; QUESADA ISABEL; CEJAS JIMENA; LUCERO AMANDA; CASTRO CLAUDIA

Vermillion. Dakota del Sur

Congreso; 18th Annual Meeting of the Nebraska Physiological Society; 2015

Nebraska Physiological Society

Background/Hypothesis: Blood vessels are surrounded by perivascular adipose tissue (PVAT) which has recently been recognized as a new factor in vascular biology. PVAT is composed mainly of adipocytes and secretes a wide range of biologically active molecules that act on the middle layer of the artery impacting on vascular smooth muscle cells (VSMC). Litlle is known about the function of PVAT in hypertension so we aim to investigate its role in the development of hypertension by analyzing PVAT proteins expression and the secretomes released by PVAT from animals with established hypertension (SHR adults) and animals that have not yet developed hypertension (pre hypertensive SHR) and compared with normotensive control animals (WKY). Methods: We first analyzed the expression of different proteins by qRT-PCR. Then we stimulated culture VSMC with PVAT secretomes and measured cell proliferation by colorimetric assay (MTT), and reactive oxygen species (ROS) generation using a fluorescent dye. Adipokine expression was determined by western blot. Results: We found that PVAT from pre hypertensive rats and SHR expressed high levels of mRNA NADPH oxidase subunits, the main oxidative stress generator in the vascular wall and low levels of visfatin expression. VSMC stimulated by secretome from SHR PVAT exhibited augmented cell proliferation and increased ROS generation. Conclusions: Our results showed that PVAT express and secretes substances that have impact on the vascular wall suggesting a possible role of this tissue in the development and maintenance of the hypertension.