IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
EXPRESSION OF NEI, TH AND TRH-R1 IN HYPOTHALAMIC AREAS OF ADULT MALE RATS: REGULATION BY THYROID HORMONES
Autor/es:
AYALA C; PENNACCHIO GE; SOAJE M; JAHN GA; SELTZER AM; CELIS ME; VALDEZ SR
Lugar:
Estancia Grande
Reunión:
Congreso; XXXII REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2014
Institución organizadora:
SOCIEDAD DE BIOLOGÍA DE CUYO
Resumen:
Neuropeptide glutamic acid-isoleucine-amide (NEI) is a 13-amino acid peptide. Both low and high levels of thyroid hormones (THs) modify NEI concentration in hypothalamic areas involved in the regulation of reproduction and certain behaviors in adult male and female rats. NEI activates the dopaminergic system inducing locomotor activity and excessive grooming behavior in the rat. In the present study we explored the effects of THs levels on NEI, Tyrosine Hydroxilase (TH, limiting enzyme of dopamine biosynthesis) and TRH Receptor 1 (TRH-R1) mRNA expression in the peduncular part of the lateral hypothalamus (PLH), preoptic area (POA) and arcuate nucleus (ARC) of male Wistar control (Co), hypothyroid (HypoT: 0.1 g/L PTU in drinking water) and hyperthyroid (HyperT: T4, 250 μg/kg/day, sc) rats, sacrificed after 21 days of treatment or vehicle administration. Hypothalamic areas were dissected and gene expression was measured by real time RT-PCR. One way ANOVA analysis was performed. PLH NEI expression increased in treated groups (p