IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of hypothyroidism on mammary function at the end of lactation in the rat
Autor/es:
CAMPO VERDE ARBOCCO FIORELLA; HAPON MB; JAHN GA
Lugar:
San Luis
Reunión:
Congreso; Reunión científica anual de la sociedad de biología de cuyo; 2014
Resumen:
Effect of hypothyroidism on mammary function at the end of lactation in the rat.Campo Verde Arboccó F, Hapon MB, Jahn GA IMBECU-CONICET-CCT-Mendoza, Argentinagjahn@mendoza-conicet.gov.arDuring lactation, prolactin (PRL) binding to its receptor (RPRL) results in activation of JAK/STAT pathway, transcription of milk proteins and inhibition of involution. Hypothyroidism (hypoT) doesn?t affect circulating PRL during lactation but alters ovarian hormone profile and milk composition and secretion. To continue investigating the mechanism through which hypoT alters mammary function, we studied its effect on day 21 (L21) of lactation, on histologic markers of involution, PRL signaling mechanisms and nuclear receptors expression, determining by real time PCR the mammary expression of PRL (prlr), estrogen (er α and β), P4 (pr) and thyroid (tr α and β) receptors, stat5b and stat5a (PRL signaling mediator), socs1, socs3 and cis (PRL signaling suppressors), α lactoalbumin (lalba) and β casein (csn2) (milk protein), ncor1, rxrα, cd1 (nuclear receptors co-regulators), bax and bcl2 (apoptotic markers). HypoT induced premature mammary involution, lowered the expression of prlr, lalba, csn2 and increased that of socs1, socs3 and erβ without affecting the expression of the rest of the studied factors, including bax and bcl2. These results suggest that hypoT may favour premature mammary involution and the fall in milk protein synthesis by repressing PRL signaling. Área temática: (BM) / Provincia de origen: Mendoza