CHRONIC HYPOTHYROIDISM ALTERS ENERGY BALANCE AND ADIPOCYTE SECRETION ACTIVITY

ORTIZ, N.C.; SANTIANO, F.; ZYLA, L.; PENNACHIO, G.; SASSO, C.V.; SOAJE, M.; CARÓN, R.W.; LÓPEZ-FONTANA, C.

San Luis

Congreso; XXXII Reunión Científica de la SBCuyo.; 2014

Sociedad de Biología de Cuyo

We investigated the effect of chronic thyroid deficiency on food intake, body weight, body fat mass and serum leptin levels in rats. Female Sprague-Dawley rats were divided into two groups at 55 days of age (Day 1): euthyroid (EUT, n=10) and hypothyroid rats (HYPO, 0.01% PTU in drinking water, n=10). All the animals were weighted weekly and food intake was assessed every other week. Blood was collected on days 1, 35 and 70 for hormone determinations. The rats were sacrificed on day 70 by decapitation. Hypothalamus, a piece of intra-abdominal and mammary fat were taken for histological and molecular analysis (ObR, Adipo R1, mTOR, POMC, NPY). Statistical analysis was performed by Student T test and Chi square (IC>95%). Food intake was lower in HYPO rats compared to EUT during the whole study (P