IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
NADPH- oxidase (NOX) and nitric oxide synthase (NOS) in high blood pressure : modulation by dietary interventions
Autor/es:
MÓNICA GALLEANO; BARBARA PIOTRKOWSKI; VALERIA CALABRÓ; MARÍA C. LITTERIO; PAULA D. PRINCE
Lugar:
Kyoto
Reunión:
Congreso; 17 th Biennal Meeting of the Society for Free Radical Research International; 2014
Resumen:
Background: NOX and NOS activities are key players to determine NO steady-state concentration, being their modulation relevant factors in the control of BP and other specific organ functions. Methods: NOX and NOS were studied in aorta, heart and kidney of rats with increased BP by: a) L-NAME (360 mg/l in beverage, 4d); and b) fructose (10% in beverage, 8wk). In both models, the effect of dietary intervention with (-)-epicatechin (EC) was analyzed. Results: In L-NAME rats, the three tissues showed increased NOX activity (2-3 times higher than control rats). As opposite, L-NAME treated rats showed a strong decrease in NOS activity (61 % of decrease respect to the control values). EC (360 mg/kg bw, 4d) avoided hypertension development in association with restoration of NOS and NOX activities to control values. In fructose-overload rats, with a moderate increase in BP, the three tissues showed both NOX and NOS activities significantly increased. EC (20 mg/kg bw, 8wk) prevented the increase in BP, in association with a restoration of NOX activity to normal values. Interestingly, NOS activity was found increased in the three tissues to values significantly higher than the corresponding to fructose-overload rats. Conclusion: Increase in NO steady-state concentration would be a central mechanism for EC action.