IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of perivascular adipose tissue in the development of hypertension.
Autor/es:
REDONDO,A; QUESADA, I; CEJAS, JIMENA; LUCERO, AMANDA; CANNIZZO, B; CASTRO CLAUDIA
Reunión:
Otro; XXXII Reunión Científica Anual De La Sociedad De Biología De Cuyo; 2014
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Blood vessels are surrounded by perivascular adipose tissue (PVAT) which has recently been recognized as a new factor in vascular biology. PVAT is composed mainly of adipocytes and secretes a wide range of biologically active molecules that act on the middle layer of the artery impacting on vascular smooth muscle cells (VSMC). Little is known about the role of PVAT in hypertension so we aim to investigate its role in the development of hypertension by analyzing the PVAT proteins expression and the secretomes released by PVAT from animals with established hypertension (SHR adults) and animals that have not yet developed hypertension (pre-SHR) and compared with normotensive control animals (WKY). We first analyzed the expression of different proteins by qRT-PCR in PVAT from three experimental groups. Then we stimulated culture VSMC from WKY control animals with PVAT secretomes and measured cell proliferation by colorimetric assay (MTT). We found that PVAT from pre hypertensive rats and SHR expressed high levels of mRNA NADPH oxidase subunits, the main oxidative stress generator in the vascular wall and low levels of mRNA adipokines. VSMC stimulated with secretome from SHR PVAT exhibited increased cell proliferation. Our results showed that PVAT express and secretes substances that have impact on the vascular wall suggesting a possible role of this tissue in the development and maintenance of the hypertension.