IMBECU   20882
INSTITUTO DE MEDICINA Y BIOLOGIA EXPERIMENTAL DE CUYO
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Thyroid disorders influence nuclear receptor comodulators gene expression in rat hypothalamus during the transition from late pregnancy to early lactation.
Autor/es:
PENNACCHIO GE; AYALA C; SOAJE M; JAHN GA; VALDEZ SR
Lugar:
Maipu
Reunión:
Congreso; XXXI Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2013
Institución organizadora:
Sociedad de Biología de Cuyo
Resumen:
Thyroid disorders impair fertility in women and cause pregnancy disorders and lactation failure. Previously we have shown that these pathologies alter thyroid hormone receptors (TR) expression at hypothalamic level. TR transcriptional activity is regulated by modulators with emerging roles in homeostasis. In this work we explore the modulation by thyroid hormones of TR repressors (NCoR) and activators (CoA) and of iodothyronine deiodinase II (DIO2) that converts locally T4 to T3. We studied the effects of hyper- and hypothyroidism on the regulation of TR pathways by determining the expression of modulators of TR activity in medial basal hypothalamus (MBH), where neuroendocrine dopaminergic neurons reside, during late pregnancy and early lactation. Wistar control (Co) hypothyroid (HypoT: 0.1 g/L PTU in drinking water), hyperthyroid (HyperT: T4, 250 µg/kg/day, sc) rats were mated 8 days after the start of treatment and sacrificed at days 19 (G19), 20 (G20), 21 (G21) of pregnancy and 2 of lactation (L2). The MBH expression of modulators was measured using real time RT-PCR. Two way ANOVA analyses were performed. In Co and HyperT rats CoA1 and CoA2 and DIO2 expression was high in G19 and tended to fall thereafter, while in HypoT rats levels were low at G19 and increased at G21 (p