ADIPOKINES AND OXIDATIVE STRESS IN PERIVASCULAR ADIPOSE TISSUE: A LINK BETWEEN DIABETES AND CARDIOVASCULAR DISEASE.

ISABEL M. QUESADA; JIMENA CEJAS; BEATRIZ CANNIZO; ANALÍA REDONDO; CLAUDIA CASTRO

Mendoza

Congreso; XXXI Reunión Anual de la Sociedad de Biología de Cuyo; 2013

Perivascular adipose tissue (PVAT) has been shown to secrete a wide variety of adipokines and produces reactive oxygen species via NADPH oxidase system. In type 2 diabetes (T2DM) PVAT-derived cytokines may influence key steps of atherogenesis. Thus, we generate two experimental models of T2DM. We fed wild type (WT) mice and apolipoprotein E-deficient mice (ApoEKO) with fructose overload (FF, 10%v/v) for 8 weeks mimicking a classical T2DM model (FF-WT) and cardiovascular complications in T2DM mice model (FF-ApoEKO). Next we analyzed plasma biochemical variables, gene expression profile of NADPH oxidase complex subunits (Nox1, Nox2, Nox4, p22phox and p47phox) and adipokines (visfatin, MCP-1 and MMP-9) in the PVAT of these two models. Biochemical variables show that glycaemia and cholesterol significantly increased in FF-WT mice compared with WT mice, while in FF-ApoEKO mice only glycaemia increased significantly versus ApoEKO control mice. In WT mice, PVAT expression of Nox1, Nox4 and p47phox genes increased significantly in the FF group. This increase was higher in FF-ApoEKO mice. In regard to markers of inflammation only MCP-1 increased significantly in FF-WT mice compared with WT. However, Visfatin, MCP-1 and MMP-9 were markedly upregulated in FF-ApoEKO mice. Our results establish a relationship between increased PVAT oxidative stress and adipokines and cardiovascular complication associated with T2DM