EFFECTS OF CADMIUM ON THE EXPRESSION OF BETA-CATENIN, CAVEOLIN-1 AND HSP27 IN ER ALPHA (+) AND ER ALPHA (-) CANCER CELL LINES

JORGE SHORTREDE; DAIANA G. ALVAREZ OLMEDO; F. DARÍO CUELLO-CARRION; DANIEL R. CIOCCA; MARIEL A. FANELLI

San Luis

Congreso; XXX REUNIÓN CIENTÍFICA ANUAL DE LA SOCIEDAD DE BIOLOGÍA DE CUYO; 2012

Sociedad de Biología de Cuyo

The metal Cd is a class 1 carcinogen associated with different human tumors. Cd induced disruption of adherents junctions mediated by E-cadherin and induced β-catenin translocation to the nucleus promoting cell proliferation and survival. In addition, there are interactions of β-catenin with Hsp27 (HSPB1) and caveolin-1 (cav-1). To evaluate the effects of Cd on the viability, cell survival, apoptosis and localization of β-catenin, Hsp27 and cav-1 in ERα(+) and ERα(-) cancer cell lines. HeLa, MDA-MB-231 and MCF-7 cells were exposed to 1-100 μM CdCl2, for 3 h. We performed MTT, clonogenic assay, immunocytochemistry, TUNEL and Bax/Bcl-2 ratio. Cd treatment reduced the viability of HeLa to 26%, MDA-MB-231 to 84% and MCF-7 to 85%. Colony forming ability was strongly inhibited in HeLa and MCF-7 and to a lesser degree in MDA-MB-231. Apoptosis was significantly increased in MCF-7. Cd differentially modulated the distribution of β-catenin, cav-1 and Hsp27 in all three cells lines. Protein location is emerging as a key factor to understand tumor cell biology.