La Pioglitazona previene el daño oxidativo e inhibe la expresión de visfatina en tejido adiposo perivascular

QUESADA, ISABEL; CEJAS, JIMENA; LOPEZ TERESA; CANNIZZO, BEATRIZ; REDONDO,ANALIA; CASTRO CLAUDIA

San Luis

Congreso; XXX Reunión Anual de la Sociedad de Biología de Cuyo; 2012

Sociedad de Biología de Cuyo

PIOGLITAZONE PREVENTS OXIDATIVE DAMAGE AND INHIBITS VISFATIN EXPRESION IN AORTIC PERIVASCULAR ADIPOSE TISSUE Quesada IM*, Cejas J#, López MT#, Cannizzo B#*, Redondo A#*, Castro C#* #Facultad de Ciencias Médicas, UNCuyo, *IMBECU, CONICET. Av. Libertador 80, (5500) Mendoza. imariaquesada@gmail.com Perivascular adipose tissue (PVAT) has been shown to secrete a wide variety of adipokines, including visfatin and produces oxygen radicals via NADPH oxidase system. Thus, PVAT provides an important site of control of vascular (dys)function in obesity and type 2 diabetes (T2DM). Pioglitazone (PIO) is an insulin sensitizer which is currently approved for treatment of T2DM. We analyzed the effect of PIO on the oxidative stress, the expression of p22 and Nox4, NADPH oxidase complex subunits, and visfatin gene expression in apolipoprotein E-deficient mice (ApoE-KO). Mice were fed with fructose overload (FF, 10% w / v) for 8 weeks and treated with PIO the latest 4 weeks We analyzed plasma biochemical variables and lipid peroxidation determined by thiobarbituric acid reactive species (TBARS). Visfatin, p22 and Nox4 gene expression were determined in ApoE-KO PVAT. Glycaemia, insulinemia, trigliceridemia and TBARS increased significantly in the FF group and PIO prevented such increases. Visfatin and FF-induced p22 and Nox4 expression were significantly diminished by PIO, in aortic PVAT. Our results show that in ApoE-KO mice, PIO improves insulin sensitivity, diminishes oxidative damage provoked by FF treatment and induced suppression of viafatin a pro-inflammatory adipocykine.