Glucose consumption and metabolic activity on lateral septum

GARCÍA S.; CASAS S.; NANFARO F.; CABRERA R.; YUNES R.

Córdoba

Congreso; XXVIICongreso Anual de la Sociedad Argentina de Investigación en Neurociencias; 2012

Sociedad Argentina de Investigación en Neurociencias

GLUCOSE CONSUMPTION AND METABOLIC ACTIVITY ON LATERAL SEPTUM S García1,2, S Casas1, F Nanfaro1, R Cabrera1, R Yunes1,2 (1) Instituto de Investigaciones Biomédicas (Universidad de Mendoza, IMBECU-CONICET) y (2) Área de Farmacología, Facultad de Ciencias Médicas (Universidad Nacional de Cuyo), Mendoza-Argentina. Glucose is one of the most important nutrients of the brain, and its consumption could be associated whit levels of brain metabolic activity. The aim of this work was to study whether or not this possible association could be of any help in order to evaluate the effect of different reactives on metabolic activity of lateral septal nucleus (LSN), our region of interest regarding memory processes. Whole LSN were incubated at 37°C with 200 μl Krebs buffer and treated with: 1) Saline; 2) Pregnenolone (Preg) 12μM; 3) Pregnenolone sulphate (PS) 12μM; 4) Lidocaine (Lid) 2 μg/μl; 5) Ap7 1 μg/μl and 6) Bicuculine (Bic) 3 μM. Glucose concentrations were measured at 0, 60 and 120 minutes. Our results showed that PS and Bic have a negative effect while Lid had a positive effect over metabolic activity. Preg and AP7 showed no effect compared to control group. This simple but effective method could be useful for several purposes, such as getting an eventual relationship of levels of neuronal activity and memory performance during diverse behavioral paradigms.